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J Bacteriol. 1962 April; 83(4): 766-774
Copyright © 1962, The Williams & Wilkins Company. All Rights Reserved.

ENZYMATIC UTILIZATION OF GLUCOSE BY A BASIDIOMYCETE

^a Northern Regional Research Laboratory,^,2 Peoria, Illinois

ABSTRACT

MELOCHE, H. P., JR. (Northern Regional Research Laboratory, Peoria, Ill.). Enzymatic utilization of glucose by a basidiomycete. J. Bacteriol. 83:766–774. 1962.—Cell-free extracts of acetone-dried Lactarius torminosus NRRL 2900 were prepared. These extracts contained hexokinase. They also contained triphosphopyridine nucleotide (TPN)-specific glucose-6-phosphate dehydrogenase and catalyzed the reduction of TPN in the presence of D-fructose-6-phosphate, 6-phospho-D-gluconic acid (6PG), and D-ribose-5-phosphate (R5P). Aged preparations oxidized D-glucose-6-phosphate (G6P) to 6PG, whereas fresh preparations oxidized G6P to a pentose with the uptake of 1 µmole of O₂ and the evolution of 1 µmole of CO₂ per µmole of G6P. Evidence for the action of transketolase in the metabolism of R5P by cell-free extracts was obtained.

Cell-free preparations lacked hexosediphosphate enzymes. Triosephosphate isomerase and F6P kinase could not be demonstrated; however, aldolase activity was present.

Evidence is presented for the conversion of D-glyceraldehyde-3-phosphate to pyruvate. In addition, phosphohexoisomerase was demonstrated.

It appears that a hexosemonophosphate pathway operates in L. torminosus extracts and may be the major mechanism of glucose dissimilation in this organism.

¹ Present address: Department of Biochemistry, Michigan State University, East Lansing.

² This is a laboratory of the Northern Utilization Research and Development Division, Agricultural Research Service, U. S. Department of Agriculture.