This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Plotz, P. H. Articles by Davis, B. D. Search for Related Content PubMed PubMed Citation Articles by Plotz, P. H. Articles by Davis, B. D.

 Previous Article  |  Next Article 

J Bacteriol. 1962 April; 83(4): 802-805
Copyright © 1962, The Williams & Wilkins Company. All Rights Reserved.

ABSENCE OF A CHLORAMPHENICOL-INSENSITIVE PHASE OF STREPTOMYCIN ACTION

Department of Bacteriology and Immunology, Harvard Medical School, Boston, Massachusetts

ABSTRACT

PLOTZ, PAUL H. (Harvard Medical School, Boston, Mass.) AND BERNARD D. DAVIS. Absence of a chloramphenicol-insensitive phase of streptomycin action. J. Bacteriol. 83:802–805. 1962.—Previous findings have suggested the possibility that the damaging effect of streptomycin on the bacterial cell membrane is only preliminary to an intracellular lethal action. Since the membrane effect requires protein synthesis, a two-stage action might be recognized if the second stage did not require protein synthesis. Indeed, it has been reported that Escherichia coli cells, pretreated with streptomycin, are not protected by chloramphenicol from subsequent killing by streptomycin in a nitrogen-free medium. However, we were unable to confirm this report or to observe any chloramphenicol-insensitive phase of streptomycin action in a growth medium. It is, therefore, not yet possible to decide whether the lethal event occurs at the membrane or within the cell.

This article has been cited by other articles:

• Davies, J., Anderson, P., Davis, B. D. (1965). Inhibition of Protein Synthesis by Spectinomycin. Science 149: 1096-1098 [Abstract]