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J Bacteriol. 1962 April; 83(4): 907-913
Copyright © 1962, The Williams & Wilkins Company. All Rights Reserved.

CONVERSION OF LACTATE-C14 TO PROPIONATE BY THE RUMEN MICROFLORA1,2

R. L. Baldwin3, W. A. Wood and R. S. Emery

a Departments of Biochemistry and Dairy, Michigan State University, East Lansing, Michigan

ABSTRACT

BALDWIN, R. L. (Michigan State University, East Lansing), W. A. WOOD, AND R. S. EMERY. Conversion of lactate-C14 to propionate by the rumen microflora. J. Bacteriol. 83:907–913. 1962.—Rumen microflora enriched on five different diets calculated to present increasing carbohydrate or lactate availability were used to determine the contribution of the randomizing (succinate) and nonrandomizing (acrylate) routes to propionate with lactate-2-C14 and -3-C14 as substrates. Propionate was labeled as though 70 to 90% was formed via the nonrandomizing route. This percentage was highest on diets containing high levels of carbohydrate or lactate or both. Evidence for the presence of succinic dehydrogenase, acetokinase, phosphotransacetylase, and coenzyme A transphorase was obtained with cell-free extracts. Propionate-2-C14 and lactate-2-C14 were converted by extracts to the activated derivatives of acrylate, lactate, propionate, and acetate.

FOOTNOTES

3 Predoctoral Fellow of the National Science Foundation.

1 Contribution no. 2904 of the Michigan Agricultural Experiment Station.

2 A summary of this work was presented at the 56th annual meeting of the American Dairy Science Association, Madison, Wis., June 11–15, 1961.

J Bacteriol. 1962 April; 83(4): 907-913
Copyright © 1962, The Williams & Wilkins Company. All Rights Reserved.





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