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J Bacteriol. 1962 May; 83(5): 1042-1046
Copyright © 1962, The Williams & Wilkins Company. All Rights Reserved.

SOME FACTORS AFFECTING STEROL FORMATION IN SACCHAROMYCES CEREVISIAE¹

^a Department of Microbiology, Oregon State University, Corvallis, Oregon

ABSTRACT

STARR, PATRICIA R. (Oregon State University, Corvallis) AND L. W. PARKS. Some factors affecting sterol formation in Saccharomyces cerevisiae. J. Bacteriol. 83:1042–1046. 1962.—A wild-type diploid strain of Saccharomyces cerevisiae was used in a study of factors that influence sterol synthesis. Maltose, glucose, sodium acetate, and ethanol were shown to be readily available for sterol synthesis in growing cultures of yeast. In cells grown anaerobically and then exposed to various substrates in aerobic resting-cell suspension, only glucose and ethanol stimulated ergosterol formation. Under these conditions, sterol synthesis was directly proportional to the amount of glucose provided. Sulfanilamide decreased the yield of sterol in growing cells, but had no effect on sterol synthesis by resting cultures.

¹ Approved for publication by the Director of the Agricultural Experiment Station as technical paper number 1484 of the journal series.