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J Bacteriol. 1962 May; 83(5): 1140-1146
Copyright © 1962, The Williams & Wilkins Company. All Rights Reserved.

ELECTRON TRANSPORT PARTICLES FROM BACILLUS STEAROTHERMOPHILUS¹

^a Department of Microbiology and Department of Chemistry, University of Nebraska, Lincoln, Nebraska

ABSTRACT

DOWNEY, RONALD J. (University of Nebraska, Lincoln), CARL E. GEORGI, AND WALTER E. MILITZER. Electron transport particles from Bacillus stearothermophilus. J. Bacteriol. 83:1140–1146. 1962—Electron-transport particles (ETP) have been isolated from Bacillus stearothermophilus. They are capable of oxidizing such substrates as succinate, malate, diphosphopyridine nucleotide, p-phenylenediamine, and hydroquinone. Difference spectra indicated bands of cytochromes a₃, b, and c. A chromatographic procedure for purification of cytochrome c has been described.

The role of quinonelike intermediates in the ETP was implied by the restorative effect of coenzyme Q and vitamin K₁ on material treated with lipid solvent. Isolation and identification of coenzyme Q from the thermophile indicated it was similar to coenzyme Q₁₀ of mammalian sources.

² Present address: Human Nutrition Research Division, Agricultural Research Service, U. S. Department of Agriculture, Beltsville, Md.

¹ A major portion of this study was presented at the 61st annual meeting of the American Society for Microbiology, Chicago, Ill., 24 April 1961.