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J Bacteriol. 1962 July; 84(1): 137-146
Copyright © 1962, The Williams & Wilkins Company. All Rights Reserved.

L-ARABINOSE-SENSITIVE, L-RIBULOSE 5-PHOSPHATE 4-EPIMERASE-DEFICIENT MUTANTS OF ESCHERICHIA COLI

Department of Biological Sciences, University of Pittsburgh, Pittsburgh, Pennsylvania
Department of Biochemistry, Michigan State University, East Lansing, Michigan

ABSTRACT

ENGLESBERG, E. (University of Pittsburgh, Pittsburgh, Pa.), R L. ANDERSON, R. WEINBERG, N. LEE, P. HOFFEE, G. HUTTENHAUER, AND H. BOYER. L-Arabinose-sensitive, L-ribulose 5-phosphate 4-epimerase-deficient mutants of Escherichia coli. J. Bacteriol. 84:137–146. 1962—L-Arabinose-negative mutants of Escherichia coli B/r, ara-53 and ara-139, are deficient in the enzyme L-ribulose 5-phosphate 4-epimerase; ara-53, further analyzed, accumulates large quantities of L-ribulose 5-phosphate when incubated with L-arabinose. The mutant sites are closely linked to the left of the previously ordered L-arabinose mutant sites, and probably represent the structural gene for L-ribulose 5-phosphate 4-epimerase (gene D) in the L-arabinose operon. The inducible levels of L-arabinose isomerase and L-ribulose 5-phosphate 4-epimerase vary correspondingly as a result of mutation in the structural gene for L-ribulokinase (gene B), further substantiating the dual structural and regulatory function of this gene locus. Ara-53 and ara-139 are strongly inhibited by L-arabinose and give rise to L-arabinose-resistant mutants. The one resistant mutant analyzed still lacks the 4-epimerase but is deficient in L-ribulokinase and has increased L-arabinose isomerase activity, a characteristic of a type of mutation in the B gene. It is proposed that accumulation of L-ribulose 5-phosphate is responsible for the inhibition, and that mutation to resistance will involve mutation in the A, B, C, permease, or repressor genes, thus providing a direct method for isolating these types of L-arabinose-negative mutants. Glucose prevents and cures the L-arabinose inhibition.

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