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J Bacteriol. 1962 July; 84(1): 23-30
Copyright © 1962, The Williams & Wilkins Company. All Rights Reserved.

METABOLIC CONTROL OF ß-GLUCOSIDASE SYNTHESIS IN YEAST

^a Department of Bacteriology, University of Wisconsin, Madison, Wisconsin

ABSTRACT

MACQUILLAN, ANTHONY M. (University of Wisconsin, Madison) AND HARLYN O. HALVORSON. Metabolic control of ß-glucosidase synthesis in yeast. J. Bacteriol. 84:23–30. 1962—The hybrid Saccharomyces fragilis x S. dobzhanskii produced a constitutive ß-glucosidase when grown in succinate synthetic medium. Upon addition of ß-glucosides, thio-ß-glucosides, or low concentrations of glucose, a further induction of enzyme synthesis was observed. Studies with other sugars revealed some specificity in response to hexose induction. Phenyl-thio-ß-D-glucoside did not affect constitutive synthesis nor induction by glucosides, thio-glucosides, or glucose. Repression of ß-glucosidase synthesis is brought about by high concentrations of glucose and other carbon compounds. Preinduction does not confer resistance to catabolic repression of enzyme synthesis; this leads to the conclusion that two sites of control for ß-glucosidase synthesis are present in yeast. Multiplicity of control is further suggested from: (i) the properties of the inducing system; (ii) semiconstitutive nature of enzyme synthesis; (iii) the repression of constitutive synthesis by glucose; (iv) the elevated derepressed rates of enzyme synthesis after glucose inhibition; and (v) the selection of a family of low constitutive mutants with variable inducibility.

¹ Present address: Division of Applied Biology, National Research Council, Ottawa, Canada.