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J Bacteriol. 1962 August; 84(2): 295-301
Copyright © 1962, The Williams & Wilkins Company. All Rights Reserved.

FRACTIONATION OF NEWCASTLE DISEASE VIRUS BY CHROMATOGRAPHY ON DIETHYLAMINOETHYL CELLULOSE

Department of Biology, Rensselaer Polytechnic Institute, Troy, New York

ABSTRACT

WILSON, DWIGHT E. (Rensselaer Polytechnic Institute, Troy, N.Y.). Fractionation of Newcastle disease virus by chromatography on diethylaminoethyl cellulose. J. Bacteriol. 84:295–301. 1962.—The L. Kansas and NK strains of Newcastle disease virus were chromatographed on diethylaminoethyl (DEAE) cellulose ion-exchange columns. L. Kansas virus eluted from DEAE columns showed one peak of hemagglutinating and infective particles. Two peaks of hemagglutinins and one peak of infective particles were observed when the NK strain was chromatographed, indicating that the stock virus contained a noninfectious hemagglutinating component. Treatment of the noninfectious particles with Genetron 113 resulted in an increase in the ratio of infective to hemagglutinating particles. The Genetron-treated noninfectious particles were also eluted from the DEAE column at a higher salt concentration than the untreated noninfectious particles. The results indicate that the Genetron treatment removes inhibitors of infectivity bound to the noninfectious virus particle.