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J Bacteriol. 1962 August; 84(2): 313-317
Copyright © 1962, The Williams & Wilkins Company. All Rights Reserved.

SEROLOGY OF AEROBIC AQUATIC ACTINOMYCETES I.

Factors Involved in Antiserum Production and In Vitro Antigen-Antibody Reactions

Department of Biology, North Texas State University, Denton, Texas

ABSTRACT

GUTHRIE, R. K. (North Texas State University, Denton), A. W. ROACH, AND J. K. FERGUSON. Serology of aerobic aquatic actinomycetes. I. Factors involved in antiserum production and in vitro antigen-antibody reactions. J. Bacteriol. 84:313–317. 1962.—The use of adjuvants permits shortening of immunization schedules, which results in improved specificity of in vitro reactions using sonically disrupted actinomycete antigenic preparations. The demonstration of specific serological reactions and of cross reactions is simplified, specificity is increased, and multiplicity of reactions is detectable by the use of agar-gel diffusion techniques in the study of individual isolates.