This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Stjernholm, R. L. Articles by Flanders, F. Search for Related Content PubMed PubMed Citation Articles by Stjernholm, R. L. Articles by Flanders, F.

 Previous Article  |  Next Article 

J Bacteriol. 1962 September; 84(3): 563-568
Copyright © 1962, The Williams & Wilkins Company. All Rights Reserved.

METABOLISM OF D-RIBOSE-1-C¹⁴ AND C¹⁴-LABELED D-GLUCONATE IN AN ENZYME SYSTEM OF THE GENUS PROPIONIBACTERIUM

Department of Biochemistry, Western Reserve University, Cleveland, Ohio

ABSTRACT

STJERNHOLM, RUNE L. (Western Reserve University, Cleveland, Ohio) AND FRANK FLANDERS. Metabolism of D-ribose-1-C¹⁴ and C¹⁴-labeled D-gluconate in an enzyme system of the genus Propionibacterium. J. Bacteriol. 84:563–568. 1962.—Ribose-1-C¹⁴ and potassium gluconate labeled in different positions were incubated with cell-free extracts of Propionibacterium shermanii. The resulting propionate, acetate, and succinate were isolated and the C¹⁴ distribution determined by degradation. It is proposed that the extensive randomization observed is caused by the conversion of the labeled substrates to fructose-6-phosphate via the transketolase-transaldolase sequence followed by the Embden-Meyerhof pathway, and that the triosephosphates produced by these metabolic routes are metabolized via pyruvate to succinate and propionate.