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J Bacteriol. 1962 October; 84(4): 735-741
Copyright © 1962, The Williams & Wilkins Company. All Rights Reserved.

SEROLOGICAL TYPING OF STAPHYLOCOCCI BY MEANS OF FLUORESCENT ANTIBODIES I.

Development of Specific Reagents for Seven Serological Factors¹

^a Communicable Disease Center, U. S. Public Health Service, Department of Health, Education, and Welfare, Atlanta, Georgia
Gade Institute, University of Bergen, Bergen, Norway

ABSTRACT

COHEN, JAY O. (Communicable Disease Center, Atlanta, Ga.) AND PER OEDING. Serological typing of staphylococci by means of fluorescent antibodies. I. Development of specific reagents for seven serological factors. J. Bacteriol. 84:735–741. 1962—Fluorescent antibody reagents for identifying seven antigenic factors of staphylococci have been prepared. The fluorescent staining reactions of these reagents were compared to the agglutination reactions with diagnostic cultures of coagulase-positive staphylococci. Correlation between the two serological tests was almost complete with factors a, b, i, and k. The c fluorescent antibody reagent had a somewhat broader spectrum of activity than the corresponding agglutination serum, whereas the m fluorescent antibody reagent stained fewer strains than were agglutinated in m serum. The fluorescent antibody reagent for h factor stained strains possessing h₁ factor but not strains possessing only h₂ factor. Fluorescent antibody reagents for specific staphylococcal factors did not stain strains of group A streptococci.

² Per Oeding was a World Health Organization Visiting Scientist at the Communicable Disease Center during September and October of 1960.

¹ Presented in part at the Annual Meeting of the Americal Society for Microbiology, Chicago, Ill., 1961.