This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Eigelsbach, H. T. Articles by White, J. D. Search for Related Content PubMed PubMed Citation Articles by Eigelsbach, H. T. Articles by White, J. D.

 Previous Article  |  Next Article 

J Bacteriol. 1962 November; 84(5): 1020-1027
Copyright © 1962, The Williams & Wilkins Company. All Rights Reserved.

LIVE TULAREMIA VACCINE I.

Host-Parasite Relationship in Monkeys Vaccinated Intracutaneously or Aerogenically¹

^a U.S. Army Chemical Corps, Fort Detrick, Frederick, Maryland

ABSTRACT

EIGELSBACH, H. T. (Fort Detrick, Frederick, Md.), J. J. TULIS, M. H. MCGAVRAN AND J. D. WHITE. Live tularemia vaccine. I. Host-parasite relationship in monkeys vaccinated intracutaneously or aerogenically. J. Bacteriol. 84:1020–1027. 1962.—Bacteriological, histological, immunohistochemical, and serological studies were made on monkeys administered live tularemia vaccine strain LVS by either of two routes. Comparative data are presented on nonvaccinated monkeys exposed via the respiratory route to a highly virulent strain of Pasteurella tularensis. Tissue changes resulting from either aerogenic or intracutaneous vaccination were mild, and consisted primarily of the proliferation of histiocytes without the formation of granulomas. The vaccine strain was isolated from the site of vaccination of animals inoculated dermally, from the lungs of animals vaccinated aerogenically, and from the regional lymph nodes, liver, and spleen of both groups; it was not isolated from the blood or bone marrow. Proliferation of the vaccine strain at the site of dermal inoculation and in the lungs of animals exposed aerogenically was observed within 24 hr; in both groups, the maximal viable population was reached within 3 days and maintained through the 10th day. A reduction in the number of viable vaccine organisms had begun by the 14th day; isolations were obtained only from the regional lymph nodes on the 28th day, and the vaccine strain was not isolated from any of the tissues cultured on the 90th day. Because the monkey is less resistant to tularemia than is man, the benign response of this animal to live tularemia vaccine indicates that the vaccine might also be safe for man when administered by either the dermal or respiratory route.

² Present address: Department of Anatomy, Washington University School of Medicine, St. Louis, Mo.

¹ Portions of this paper were presented at the 62nd Annual Meeting of the American Society for Microbiology, Kansas City, Mo., May, 1962.

This article has been cited by other articles:

• RICK LYONS, C., WU, T. H. (2007). Animal Models of Francisella tularensis Infection. Ann. N. Y. Acad. Sci. 1105: 238-265 [Abstract] [Full Text]  
• Nix, E. B., Cheung, K. K. M., Wang, D., Zhang, N., Burke, R. D., Nano, F. E. (2006). Virulence of Francisella spp. in Chicken Embryos.. Infect. Immun. 74: 4809-4816 [Abstract] [Full Text]  
• Wu, T. H., Hutt, J. A., Garrison, K. A., Berliba, L. S., Zhou, Y., Lyons, C. R. (2005). Intranasal Vaccination Induces Protective Immunity against Intranasal Infection with Virulent Francisella tularensis Biovar A. Infect. Immun. 73: 2644-2654 [Abstract] [Full Text]  
• Ellis, J., Oyston, P. C. F., Green, M., Titball, R. W. (2002). Tularemia. Clin. Microbiol. Rev. 15: 631-646 [Abstract] [Full Text]