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J Bacteriol. 1962 November; 84(5): 1094-1098
Copyright © 1962, The Williams & Wilkins Company. All Rights Reserved.

FORMATION OF PROTOPLASTS FROM STREPTOCOCCUS FAECALIS BY LYSOZYME1

William R. Bibb and W. R. Straughn

a Department of Bacteriology and Immunology, School of Medicine, University of North Carolina, Chapel Hill, North Carolina

ABSTRACT

BIBB, WILLIAM R. (University of North Carolina, Chapel Hill) AND W. R. STRAUGHN. Formation of protoplasts from Streptococcus faecalis by lysozyme. J. Bacteriol. 84:1094–1098. 1962.—Incubation of whole cells of Streptococcus faecalis F24 in the presence of the crystalline egg-white lysozyme and appropriate sucrose concentration resulted in the formation of discrete spherical structures. On dilution, these osmotically fragile structures lysed immediately. Methyl pentose determinations on isolated cell walls and protoplast membranes verified the presence of rhamnose in the cell walls and its essentially complete absence in protoplast membranes. Cell walls were rendered soluble by lysozyme. After lysozyme treatment of cell walls, 96% of the rhamnose present was not sedimented by centrifugation at 12,500 x g for 30 min. No cell-wall structures were recognized by phasecontrast or electron microscopy. After direct lysis of whole cells of S. faecalis F24 by lysozyme, protoplast membranes were isolated. It is concluded that, in the strain of group D streptococcus studied, lysozyme effectively removes the cell wall.

FOOTNOTES

1 This work was presented in part at the 62nd Annual Meeting of the American Society for Microbiology, Kansas City, Mo., May, 1962.

J Bacteriol. 1962 November; 84(5): 1094-1098
Copyright © 1962, The Williams & Wilkins Company. All Rights Reserved.





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