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J Bacteriol. 1962 November; 84(5): 1104-1110
Copyright © 1962, The Williams & Wilkins Company. All Rights Reserved.

HEMATIN-REQUIRING PLASMODIAL MYXOMYCETE

McArdle Memorial Laboratory, University of Wisconsin, Madison, Wisconsin

ABSTRACT

DANIEL, JOHN W. (University of Wisconsin, Madison), JACQUELINE KELLEY, AND HAROLD P. RUSCH. Hematin-requiring plasmodial myxomycete. J. Bacteriol. 84:1104–1110. 1962.—The myxomycete Physarum polycephalum, previously shown to require chick embryo extract for growth on a partially defined, soluble medium, grows as well if hematin or certain hemoproteins are substituted for the embryo extract. Hematin is also required as a growth factor if the organism is grown on a synthetic medium. Of the variety of porphyrins tested only iron protoporphyrin IX is utilized for growth by P. polycephalum. Protoporphyrin IX is inactive. Protein-bound iron porphyrin is active at one-tenth the concentration of free hematin. Although hematin completely replaces embryo extract, the extract activity has properties not characteristic of hematin or the hemoproteins tested: ladility to light and rapid plasmodial uptake.