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INDUCTION OF LYSOGENIC MICROCOCCUS LYSODEIKTICUS¹

^a Division of Bacteriology, College of Agriculture, Cornell University, Ithaca, New York

ABSTRACT

FIELD, A. K. (Cornell University, Ithaca, N.Y.) AND H. B. NAYLOR. Induction of lysogenic Micrococcus lysodeikticus. J. Bacteriol. 84:1129–1133. 1962.—Between 50 and 60% of log-phase cells of Micrococcus lysodeikticus strain ML 53-40, lysogenic for N5 bacteriophage, were induced by an optimal dose of ultraviolet light. Induction of dilute cell suspensions caused a subsequent 200- to 800-fold increase in infective-center concentration. Cells in the stationary growth phase and cells adapted to a chemically defined medium were also induced by ultraviolet irradiation. Dimethyl sulfate induced the lysogenic culture to about the same extent as did ultraviolet light, whereas ß-propiolactone was less effective.

² Present address: Department of Bacteriology, University of California, Berkeley.

¹ Taken in part from a dissertation submitted by the senior author to the Graduate Faculty of Cornell University, in partial fulfillment of the requirements for the degree of Master of Science.