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USE OF POROUS CELLULOSE ESTER MEMBRANES IN THE PRIMARY ISOLATION AND SIZE DETERMINATION OF PLEUROPNEUMONIA-LIKE ORGANISMS

Department of Biophysics, Yale University, New Haven, Connecticut
Department of Bacteriology, University of Connecticut, Storrs, Connecticut

ABSTRACT

MOROWITZ, HAROLD J. (Yale University, New Haven, Conn.), MARK E. TOURTELLOTTE, AND MARY E. POLLACK. Use of porous cellulose ester membranes in the primary isolation and size determination of pleuropneumonia-like organisms. J. Bacteriol. 85:134–136. 1963.—Millipore filters were used in the primary isolation and sizing of a number of strains of pleuropneumonia-like organisms (PPLO). Maximal pore diameters were checked by filtration of viruses of known sizes. All the PPLO strains studied produced cells capable of passing through a 0.22-µ filter. A number of strains produced forms which could be titered after filtration through a 0.1-µ filter. A number of primary isolations were made from filtrates.