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GENETIC TRANSFER OF THE ABILITY TO GROW AT 55 C IN BACILLUS SUBTILIS¹

^a U.S. Army Chemical Corps Biological Laboratories, Fort Detrick, Frederick, Maryland

ABSTRACT

MCDONALD, WILLIAM C. (U.S. Army Chemical Corps, Fort Detrick, Frederick, Md.) AND THOMAS S. MATNEY. Genetic transfer of the ability to grow at 55 C in Bacillus subtilis. J. Bacteriol. 85:218–220. 1962.—Two strains of Bacillus subtilis, 168 and P1, were found to grow at 55 C (55⁺) on complete media; strain 168S^r failed to grow at temperatures above 50 C (55^–). When 168S^r bacteria were grown in the presence of deoxyribonucleic acid extracted from 168, the ability to grow at 55 C was transformed at a frequency of 10^–4. An incubation period of 3 to 4 hr at 37 C was necessary for phenotypic expression of the 55⁺ character. Only 10 to 20% of the 55⁺ transformants retained the high-level streptomycin resistance (S^r) of the recipient, indicating close linkage between the S^s and 55⁺ loci.

² Present address: Department of Bacteriology and Public Health, Washington State University, Pullman, Wash.

³ Present address: Genetics Section, The University of Texas, M. D. Anderson Hospital and Tumor Institute, Texas Medical Center, Houston Texas.

¹ This work was presented at the 62nd Annual Meeting of the American Society for Microbiology, Kansas City, Mo., May, 1962.