This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Puziss, M. Articles by Wright, G. G. Search for Related Content PubMed PubMed Citation Articles by Puziss, M. Articles by Wright, G. G.

STUDIES ON IMMUNITY IN ANTHRAX X.

Gel-Adsorbed Protective Antigen for Immunization of Man

U.S. Army Biological Laboratories, Fort Detrick, Frederick, Maryland

ABSTRACT

PUZISS, MILTON (Fort Detrick, Frederick, Md.) and GEORGE G. WRIGHT. Studies on immunity in anthrax. X. Gel-adsorbed protective antigen for immunization of man. J. Bacteriol. 85:230–236. 1963.—Protective antigen in filtrates of anaerobic cultures of Bacillus anthracis was not readily precipitable by alum or other aluminum salts, but was adsorbed onto a preformed aluminum hydroxide gel under specific conditions. The adsorbed product was highly effective in immunizing rabbits, guinea pigs, and mice against challenge with virulent B. anthracis spores. Gel-adsorbed anaerobic antigens preserved with 1:10,000 thimerosal were unstable on storage at 4 C; replacement of this preservative with 1:40,000 benzethonium chloride produced a more stable product. Addition of 0.0009% formalin further increased the stability during accelerated aging at 37 C. The final product had high protective activity in animals and was well tolerated in man.

This article has been cited by other articles:

• Grabenstein, J. D., Pittman, P. R., Greenwood, J. T., Engler, R. J.M. (2006). Immunization to Protect the US Armed Forces: Heritage, Current Practice, and Prospects. Epidemiol Rev 28: 3-26 [Abstract] [Full Text]  
• Hashimoto, M., Boyer, J. L., Hackett, N. R., Wilson, J. M., Crystal, R. G. (2005). Induction of Protective Immunity to Anthrax Lethal Toxin with a Nonhuman Primate Adenovirus-Based Vaccine in the Presence of Preexisting Anti-Human Adenovirus Immunity. Infect. Immun. 73: 6885-6891 [Abstract] [Full Text]  
• Williamson, E. D., Hodgson, I., Walker, N. J., Topping, A. W., Duchars, M. G., Mott, J. M., Estep, J., LeButt, C., Flick-Smith, H. C., Jones, H. E., Li, H., Quinn, C. P. (2005). Immunogenicity of Recombinant Protective Antigen and Efficacy against Aerosol Challenge with Anthrax. Infect. Immun. 73: 5978-5987 [Abstract] [Full Text]