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ISOLATION AND PROPERTIES OF AN EXOCELLULAR NUCLEASE OF SERRATIA MARCESCENS

^a Department of Microbiology, Wayne State University College of Medicine, Detroit, Michigan

ABSTRACT

EAVES, GEORGE N. (Wayne State University College of Medicine, Detroit, Mich.) AND CHARLES D. JEFFRIES. Isolation and properties of an exocellular nuclease of Serratia marcescens. J. Bacteriol. 85:273–278. 1963.—The exocellular nuclease of Serratia marcescens, isolated by anion-exchange chromatography on diethylaminoethyl-Sephadex, depolymerized deoxyribonucleic acid, ribonucleic acid, and the polynucleotide which is refractory to pancreatic ribonuclease activity. The enzyme was tentatively classified as a nonspecific phosphodiesterase. Magnesium was essential for activity, which was optimal at pH 8.8. The purified enzyme was completely inactivated by heating at 50 C for 15 min.

¹ Present address: Department of Biology, Washington and Jefferson College, Washington, Pa. Predoctoral trainee, Training Program in Microbiology 2G-599, Division of General Medical Science, National Institutes of Health, U.S. Public Health Service, during the terminal period of the investigation.

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