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RELATIONSHIP OF THE HEXOSE MONOPHOSPHATE SHUNT TO THE ENDOGENOUS METABOLISM OF CELL-FREE EXTRACTS OF MYCOBACTERIUM PHLEI

The Lilly Research Laboratories, Eli Lilly and Company, Indianapolis, Indiana

ABSTRACT

SUTTON, W. B. (The Lilly Research Laboratories, Indianapolis, Ind.). Relationship of the hexose monophosphate shunt to the endogenous metabolism of cell-free extracts of Mycobacterium phlei. J. Bacteriol. 85:476–484. 1963.—The endogenous reduction of 2,6-dichlorophenol-indophenol (DPIP) by cell-free extracts of Mycobacterium phlei has been linked to the presence of glucose-6-phosphate (G-6-P) dehydrogenase functioning in connection with a reduced triphosphopyridine nucleotide (TPN)-DPIP reductase. The necessary substrate and coenzyme, i.e., G-6-P and TPN, are contained in the cell-free bacterial extract. The only required addition to activate the system is a suitable electron acceptor. The accumulation of G-6-P and the presence of 6-phosphogluconic dehydrogenase in the cell-free extract suggest that the hexose monophosphate shunt mechanism is impaired by sonic treatment of M. phlei.