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MORPHOLOGY OF HUMAN GENITAL "T-STRAIN" PLEUROPNEUMONIA-LIKE ORGANISMS

Department of Medicine, University of British Columbia, Vancouver, British Columbia, Canada

ABSTRACT

FORD, DENYS K. (University of British Columbia, Vancouver, Canada), AND JOYCE MACDONALD. Morphology of human genital "T-strain" pleuropneumonia-like organisms. J. Bacteriol. 85:649–653. 1963.—The morphology of Shepard's "T-strain" organisms from the human genital tract was investigated. The incubation of "T-strains" in 20% CO₂ with 80% nitrogen for 4 to 5 days caused surface outgrowth from the central core of the colonies embedded in the agar. Filtration through HA, PH, and VC Millipore filters showed that the elementary particles of "T-strains" were between 0.3 and 0.1 µ in diameter. "T-strain" pleuropneumonia-like organisms (PPLO) from broth cultures, stained by Giemsa's method, were seen to be minute, spherical particles, similar in size to the particles of the large-colony strains. Colonies of "T-strains," when prepared by the microculture, fixed-agar-block, and Formvar methods, resembled those of large-colony PPLO. It was concluded that "T-strain" organisms were true PPLO.