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DEGRADATION OF ERGOTHIONEINE BY CELL-FREE EXTRACTS OF ALCALIGENES FAECALIS II.

Production of Glutamic Acid¹

^a Department of Bacteriology, University of Southern California, Los Angeles, California

ABSTRACT

BOOTH, JAMES S. (University of Southern California, Los Angeles) AND MILO D. APPLEMAN. Degradation of ergothioneine by cell-free extracts of Alcaligenes faecalis. II. Production of glutamic acid. J. Bacteriol. 85:654–657. 1963.—On the basis of oxidation and paper chromatographic procedures, glutamic acid was identified as the end product of ergothioneine degradation by cell-free extracts of Alcaligenes faecalis. Hydrogen sulfide and ammonia yields were determined. Several differences between the metabolism of whole cells and cell-free extracts were noted. Cleavage of the imidazole ring by cell-free extracts appeared to be hydrolytic rather than oxidative.

¹ Confirmation of the identification of this organism was recently made by Rudolph Hugh, The George Washington University, Washington, D.C. Dr. Hugh is maintaining this organism as Alcaligenes faecalis 1848.