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SHORT-TERM N₂¹⁵-INCORPORATION BY AZOTOBACTER¹

^a Charles F. Kettering Research Laboratory, Yellow Springs, Ohio

ABSTRACT

BULEN, W. A. (Charles F. Kettering Research Laboratory, Yellow Springs, Ohio), J. R. LECOMTE, AND H. E. BALES. Short-term N₂¹⁵-incorporation by Azotobacter. J. Bacteriol. 85:666–670. 1963.—Short-term N₂¹⁵-incorporation measurements were used to determine which of the growth requirements were necessary for nitrogen fixation by Azotobacter agilis (A. vinelandii). Normal cells required neither added iron nor molybdenum, but a marked stimulation by Na⁺ and a minor stimulation by Mg²⁺ were observed. The Na⁺ stimulation was not accompanied by an increase in O₂ uptake. A lag period preceded the response of molybdenum-deficient cells to added Mo. In systems employing 10 and 20% O₂ with 10% N₂¹⁵ in the gas phase, O₂ appeared to be both required and inhibitory. These observations may be helpful in attempts to fractionate cell-free nitrogen-fixing systems from this aerobe.

¹ Publication no. 103 from the Charles F. Kettering Research Laboratory, Yellow Springs, Ohio.