a Institute of Microbiology, Rutgers, The State University, New Brunswick, New Jersey
ABSTRACT
NICKERSON, WALTER J. (Rutgers, The State University, New Brunswick, N.J.) AND GIUSEPPE FALCONE. Enzymatic reduction of selenite. J. Bacteriol. 85:763771.1963. Extraction of boiled yeast extract with n-hexane removed its ability to restore selenite-reducing capacity in dialyzed enzyme preparations obtained from baker's yeast or from Candida albicans. Menadione or thiodione substituted for the quinone(s) extracted by n-hexane. Active components of the water-soluble, dialyzable fraction of yeast extract included: glucose-6-phosphate, triphosphopyridine nucleotide, and glutathione. Selenite appears to be bound to protein through vicinal thiol groups, and to be released therefrom as metallic selenium after accepting four electrons.
1 Present address: Istituto di Patologia Generale, Università di Napoli, Naples, Italy.
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