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ISOLATION, ENUMERATION, AND CHARACTERISTICS OF PROTEOLYTIC RUMINAL BACTERIA¹

^a Departments of Biology, Biochemistry and Nutrition, and of Veterinary Science, Virginia Polytechnic Institute, Blacksburg, Virginia

ABSTRACT

FULGHUM, ROBERT S. (Virginia Polytechnic Institute, Blacksburg) AND W. E. C. MOORE. Isolation, enumeration, and characteristics of proteolytic ruminal bacteria. J. Bacteriol. 85:808–815. 1963.—Colony counts of proteolytic ruminal bacteria in the order of 10⁹ organisms per g of whole rumen contents, and total colony counts in the order of 2 to 3 x 10⁹ organisms per g, were obtained from rumen contents of cattle fed a maintenance ration of hay and grain. The proteolytic counts averaged 38% of the total counts. An anaerobic, differential medium characterizing proteolytic colonies by clear zones in an opaque skim-milk suspension was utilized. Proteolytic isolates were assigned to the following taxa: Butyrivibrio sp., Succinivibrio sp., Selenomonas ruminantium var. lactilytica, Borrelia sp., Bacteroides sp., and selenomonadlike organisms similar to the B-385 group of Bryant.

² Present address: Division of Natural Sciences, Susquehanna University, Selinsgrove, Pa.

¹ A portion of a Ph.D. dissertation by the senior author.

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