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EFFECTS OF CARBON DIOXIDE ON THE COLONIAL GROWTH OF PATHOGENIC LEPTOSPIRAE

^a National Institute of Animal Health, Kodaira, Tokyo, Japan

ABSTRACT

YANAGAWA, R. (National Institute of Animal Health, Kodaira, Tokyo, Japan), T. HIRAMUNE, AND J. FUJITA. Effects of carbon dioxide on the colonial growth of pathogenic leptospirae. J. Bacteriol. 85:875–880. 1963.—The growth of colonies of pathogenic leptospirae was examined in air of various CO₂ concentrations, ranging from air from which CO₂ was absorbed to air with 20% CO₂ added, and was compared with the growth of other microorganisms of the genera Mycoplasma, Brucella, Vibrio, Erysipelothrix, Staphylococcus, Micrococcus, Sarcina, Klebsiella, Salmonella, Escherichia, and Bacillus. The colonial growth of the 24 strains of pathogenic leptospirae examined was similar to that of other organisms in that the growth was negative in air from which naturally contained CO₂ had been absorbed, the growth tended to be inhibited in 5 to 20% CO₂ in proportion to the CO₂ concentration, and good colonial growth of leptospirae was obtained in a relatively narrow range of CO₂ concentration (from the normal amount in air to around 1%). The growth of some leptospirae was accelerated in 1% CO₂, but not that of the strains of Leptospira icterohaemorrhagiae, which required a lower range of CO₂, and of L. canicola. The CO₂ requirement of pathogenic leptospirae was similar, to some extent, to that of Mycoplasma mycoides, but was not so high as that of Brucella abortus. Incorporation of C¹⁴O₂ was demonstrated in leptospirae.

¹ Present address: Faculty of Veterinary Medicine, Hokkaido University, Sapporo, Hokkaido, Japan.