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J Bacteriol. 1963 May; 85(5): 1056-1060
Copyright © 1963, The Williams & Wilkins Company. All Rights Reserved.

FATTY ACID COMPOSITION OF LIPIDS FROM STREPTOCOCCUS CREMORIS AND STREPTOCOCCUS LACTIS VAR. MALTIGENES¹

^a Department of Animal Industries, University of Connecticut, Storrs, Connecticut

ABSTRACT

MACLEOD, PATRICIA (University of Connecticut, Storrs) AND JOSEPH P. BROWN. Fatty acid composition of lipids from Streptococcus cremoris and Streptococcus lactis var. maltigenes. J. Bacteriol. 85:1056–1060. 1963.—A method for the extraction of lipids from bacterial cells, involving grinding in a ball mill, is described. Cells of Streptococcus lactis var. maltigenes and S. cremoris yielded 5.3 and 5.5%, respectively, of their dry cellular weights as lipid. The lipids of both organisms were separated into three fractions by thin-layer chromatography. Two of these fractions have been classified: one fraction containing phospho- and perhaps glycolipids and the other containing triglycerides. The unidentified fraction was probably either mono- or diglyceride in nature. The fatty acid composition of the total lipid as well as that of each of the separated lipid components from the two lactic strains were compared.

¹ Scientific Contribution No. 14, Agricultural Experiment Station, University of Connecticut, Storrs.