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J Bacteriol. 1963 May; 85(5): 983-985
Copyright © 1963, The Williams & Wilkins Company. All Rights Reserved.

NUTRITION OF LEPTOSPIRA POMONA III.

Calcium, Magnesium, and Potassium Requirements

^a U.S. Army Biological Laboratories, Fort Detrick, Frederick, Maryland

ABSTRACT

JOHNSON, R. C. (Fort Detrick, Frederick, Md.) AND N. D. GARY. Nutrition of Leptospira pomona. III. Calcium, magnesium, and potassium requirements. J. Bacteriol. 85:983–985. 1963.—The cation requirements for Leptospira pomona Wickard were investigated employing a basal medium prepared with 10% deionized rabbit serum and containing 10^–3M NH₄Cl, 5 µg/ml thiamine, and 0.01 M Na₂HPO₄ buffer (pH 7.4). Rabbit serum was deionized by passage through a column of Amberlite IRC-50 resin. This basal medium did not support growth when either Ca⁺⁺ or Mg⁺⁺ was added to it. However, when both Ca⁺⁺ (3 x 10^–5M) and Mg⁺⁺ (5 x 10^–6M) were present, growth was obtained. Although an absolute requirement for K⁺ could not be demonstrated, an increase in the amount of growth did occur if K⁺ (5 x 10^–5M) was incorporated in addition to Ca⁺⁺ and Mg⁺⁺. When adequate concentrations of Ca⁺⁺ and K⁺ were present with a limiting concentration of Mg⁺⁺ (less than 5 x 10^–7M), filamentous cells were observed.

¹ Present address: Department of Microbiology, University of Minnesota, Minneapolis.