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INDUCTION AND REPRESSION OF L-ARABINOSE ISOMERASE IN PEDIOCOCCUS PENTOSACEUS¹

^a Department of Microbiology, University of Illinois, Urbana, Illinois

ABSTRACT

DOBROGOSZ, WALTER J. (University of Illinois, Urbana) AND RALPH D. DEMOSS. Induction and repression of L-arabinose isomerase in Pediococcus pentosaceus. J. Bacteriol. 85:1350–1355. 1963.—The inducible L-arabinose isomerase of Pediococcus pentosaceus can be rapidly and conveniently measured in whole-cell preparations by use of a standard colorimetric procedure originally developed for studies with cell-free enzyme preparations. The enzyme is measured by its ability to catalyze the isomerization of L-arabinose to L-ribulose. Whole cells suspended in a suitable buffer and pretreated with toluene were shown to exhibit this isomerase activity at a level comparable with that observed in cell-free enzyme preparations. Conditions for optimal induction of L-arabinose isomerase are described. In addition, it was determined that the formation of this enzyme is subject to repression by glucose, i.e., via catabolite repression.

² Present address: Department of Botany and Bacteriology, North Carolina State College, Raleigh.

¹ This investigation was carried out during the tenure of a Postdoctoral Fellowship from the National Institute of Allergy and Infectious Diseases, U.S. Public Health Service.