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CULTURE MEDIUM FOR THE PRODUCTION OF STAPHYLOCOCCAL ENTEROTOXIN A

Division of Microbiology, Food and Drug Administration, U.S. Department of Health, Education, and Welfare, Washington, D.C.

ABSTRACT

CASMAN, E. P. (U.S. Food and Drug Administration, Washington, D.C.) AND R. W. BENNETT. Culture medium for the production of staphylococcal Enterotoxin A. J. Bacteriol. 86:18–23. 1963.—By use of Wadsworth's modification of the gel diffusion test of Ouchterlony for the assay of enterotoxin, cultivation on semisolid Brain Heart Infusion Agar of pH 5.3 permitted the detection of the production of staphylococcal Enterotoxin A with all of 49 strains without concentration of their toxins. The production of appreciably more potent enterotoxin was effected by using a cellophane sac culture technique. The use of the latter procedure, together with Brain Heart Infusion broth, permitted the detection of the production of trace amounts of Enterotoxins A and B.