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PRODUCTION OF LIPASE BY PSEUDOMONAS FRAGI IN A SYNTHETIC MEDIUM

Meat Laboratory, Eastern Utilization Research and Development Division, Agricultural Research Service, U.S. Department of Agriculture, Beltsville, Maryland

ABSTRACT

ALFORD, JOHN A. (U.S. Department of Agriculture, Beltsville, Md.) AND DAVID A. PIERCE. Production of lipase by Pseudomonas fragi in a synthetic medium. J. Bacteriol. 86:24–29. 1963.—The growth and production of lipase by Pseudomonas fragi in 41 dehydrated media were determined. From a chemical analysis of the peptone medium which gave the maximal lipase production, numerous synthetic media were prepared. Good cell growth, but no lipase production, was obtained on a buffered ammonium sulfate-glucose medium. The addition of arginine, lysine, aspartic acid, and glutamic acid to this medium gave lipase production approaching that obtained in the peptone medium. Omission of the glucose had little effect on growth, but no lipase were produced. Sterilization of the glucose with either the amino acids or phosphate buffer had no effect on growth, but sharply reduced lipase production. Gas chromatographic analysis of the fatty acids liberated from lard, corn oil, and coconut oil indicated that medium composition had no effect on enzyme specificity. P. fluorescens produced very little lipase in any of the synthetic media examined.