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TERMINAL OXIDATION IN CELL-FREE EXTRACTS OF FUNGI¹

^a Department of Plant Pathology, University of Illinois, Urbana, Illinois

ABSTRACT

DOWLER, WILLIAM M. (University of Illinois, Urbana), PAUL D. SHAW, AND DAVID GOTTLIEB. Terminal oxidation in cell-free extracts of fungi. J. Bacteriol. 86:9–17. 1963.—The terminal respiration in cell-free extracts of ten representative fungi is mediated by an electron transport system similar to that observed in animal tissue. Reduced nicotinamide adenine dinucleotide (NADH) and succinic cytochrome c reductases and NADH oxidase activity are contained in the extracts. An antimycin-sensitive site and cytochrome oxidase are present. Dehydrogenases including glucose-6-phosphate, triose phosphate, isocitric, glutamic, succinic, and malic dehydrogenase were found, but pyruvic and -ketoglutaric dehydrogenases were absent. The soluble nature of the dehydrogenases indicates probable disruption of the mitochondria, since normally these enzymes are found within the mitochondria. Particles containing most of the terminal respiratory activity could be sedimented by centrifugation at 40,000 x g for 30 min. Oxygen was utilized by cell-free extracts with NADH, succinate, and isocitrate as substrates, but not with glucose.

² Present address: Crops Research Division, Agricultural Research Service, U.S. Department of Agriculture, Department of Botany and Bacteriology, Clemson College, Clemson, S.C.

¹ This report is from a dissertation submitted by William M. Dowler in partial fulfillment of the requirements for the Ph.D. degree in the Graduate School of the University of Illinois.