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SPORE GERMINATION AND CARBON METABOLISM IN FUSARIUM SOLANI IV.

Metabolism of Ethanol and Acetate

^a Department of Biology, Wesleyan University, Middletown, Connecticut

ABSTRACT

COCHRANE, VINCENT W. (Wesleyan University, Middletown, Conn.), JEAN C. COCHRANE, JAMES M. VOGEL, AND ROSWELL S. COLES, JR. Spore germination and carbon metabolism in Fusarium solani. IV. Metabolism of ethanol and acetate. J. Bacteriol. 86:312–319. 1963.—The aerobic metabolism of acetate and ethanol by ungerminated spores of Fusarium solani f. phaseoli was accompanied by oxidative assimilation, respectively, of 50 and 75% of the substrate carbon. Manometric and isotope distribution studies suggested terminal oxidation by way of the tricarboxylic acid and glyoxylate cycles. Provision of exogenous ammonium ion had no effect. The endogenous respiration of germinated spores was depressed by ethanol. Malonate and acetoin were oxidized in a way consistent with a pathway via acetate. Acetate oxidation was inhibited by cyanide, azide, 2,4-dinitrophenol, fluoroacetate, and other common inhibitors. Acetate was assimilated primarily into compounds extractible with hot water, of which mannitol was the major component, and was not appreciably incorporated into spore lipids. Ethanol, but not acetate, accelerated the oxidation of glucose by mechanisms not now understood.

¹ Present address: The Mount Sinai Hospital, New York, N.Y.

² Present address: Department of Microbiology, University of Minnesota, Minneapolis.