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ISOLATION AND PROPERTIES OF A PUTRESCINE-DEGRADING MUTANT OF ESCHERICHIA COLI

Department of Chemistry, Wayne State University, Detroit, Michigan

ABSTRACT

KIM, KI-HAN (Wayne State University, Detroit, Mich.). Isolation and properties of a putrescine-degrading mutant of Escherichia coli. J. Bacteriol. 86:320–323. 1963.—A mutant was isolated from Escherchia coli B which can grow on putrescine as the sole source of carbon and nitrogen. This mutant contains a constitutive putrescine -ketoglutarate transaminase and can be induced for the enzymes responsible for the conversion of -aminobutyraldehyde to succinate. The intracellular putrescine of this mutant could be nearly quantitatively removed by nitrogen starvation. This removal of intracellular putrescine did not damage the reproduction of T₂ in these cells.

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