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INCORPORATION OF DEOXYRIBONUCLEIC ACID IN THE BACILLUS SUBTILIS TRANSFORMATION SYSTEM¹

^a Department of Microbiology, Western Reserve University, Cleveland, Ohio
Department of Pathology, Western Reserve University, Cleveland, Ohio

ABSTRACT

YOUNG, F. E. (Western Reserve University Cleveland, Ohio) AND JOHN SPIZIZEN. Incorporation of deoxyribonucleic acid in the Bacillus subtilis transformation system. J. Bacteriol. 86:392–400. 1963.—The optimal conditions for the incorporation of deoxyribonucleic acid (DNA) were studied. In competent cells, the irreversible binding of DNA was influenced by temperature, hydrogen ion concentration, and aeration. Divalent cations, such as barium, strontium, calcium, or magnesium, were required. Under suboptimal environmental conditions and with metabolic inhibitors, the process of transformation was decreased to a greater extent than was incorporation of DNA. Under conditions of phosphate depletion, the incorporation of P³² increased. However, the frequency of transformation decreased. This inducible process was not related to competence.

² Faculty Research Associate, American Cancer Society. Present address: Department of Pathology, Western Reserve University, Cleveland, Ohio.

³ Present address: Department of Microbiology, University of Minnesota, Minneapolis.

¹ Portion of a thesis submitted in partial fulfillment of the requirements for a Ph.D. degree in Microbiology at Western Reserve University. A portion of this study was presented at the annual meeting of the Federation of American Societies for Experimental Biology, Atlantic City, 1961.

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