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ALTERATION IN TRANSFORMABILITY OF DIPLOCOCCUS PNEUMONIAE AFTER THE ACQUISITION OF GENETIC DETERMINANTS INDUCING RESISTANCE TO ERYTHROMYCIN

Division of Experimental Chemotherapy, Sloan-Kettering Institute for Cancer Research, and Sloan-Kettering Division, Graduate School of Medical Sciences, Cornell University Medical College, New York, New York

ABSTRACT

SIROTNAK, FRANCIS M. (Sloan-Kettering Institute for Cancer Research, New York, N.Y.), RAMONA B. LUNT, AND DORRIS J. HUTCHISON. Alteration in transformability of Diplococcus pneumoniae after the acquisition of genetic determinants inducing resistance to erythromycin. J. Bacteriol. 86:735–739. 1963.—The genetic alteration of a highly transformable (competent) recipient strain of Diplococcus pneumoniae by the transformation of at least two of three identified erythromycin-resistance determinants (Ery_a, Ery_b, Ery_c) results in a marked decrease (Ery_a-b recombinant) or complete loss (Ery_a-c recombinant) in the ability to be transformed. The occurrence of transformable cells in cultures of the R6 Ery_a-b recombinant strains, although greatly diminished, still varies during growth in a manner characteristic of the fully competent parent strain. Both Ery_b and Ery_c determinants appear to be linked to Ery_a. In experiments using P³²-labeled deoxyribonucleic acid (DNA), data correlating DNA uptake with transformation show a decrease or loss in uptake capacity of the erythromycin-resistant strains.