This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Weimberg, R. Articles by Orton, W. L. Search for Related Content PubMed PubMed Citation Articles by Weimberg, R. Articles by Orton, W. L.

REPRESSIBLE ACID PHOSPHOMONOESTERASE AND CONSTITUTIVE PYROPHOSPHATASE OF SACCHAROMYCES MELLIS¹

^a Pioneering Laboratory for Microbiological Chemistry, Northern Regional Research Laboratory, U.S. Department of Agriculture, Peoria, Illinois

ABSTRACT

WEIMBERG, RALPH (Northern Regional Research Laboratory, Peoria, Ill.), AND WILLIAM L. ORTON. Repressible acid phosphomonoesterase and constitutive pyrophosphatase of Saccharomyces mellis. J. Bacteriol. 86:805–813. 1963.—Saccharomyces mellis produces a nonspecific acid phosphomonoesterase (pH optimum of 5.5 to 6.0) when grown in a medium devoid of phosphate. Only minimal amounts of this enzyme are present in cells harvested from media containing phosphate. The enzyme requires no cofactors. It is inhibited by such anions as phosphate, arsenate, molybdate, and borate. S. mellis also contains an inorganic pyrophosphatase with a pH optimum of 7.5. The properties of this enzyme are distinctly different from those of the acid phosphomonoesterase. The pyrophosphatase requires Mg⁺⁺ for activity. This enzyme is constitutive, since it is present in cells regardless of the phosphate content of the growth medium.

¹ Presented at the Annual Meeting of the American Society for Microbiology, Cleveland, Ohio, 5 to 9 May 1963.

This article has been cited by other articles:

• Obojska, A., Ternan, N. G., Lejczak, B., Kafarski, P., McMullan, G. (2002). Organophosphonate Utilization by the Thermophile Geobacillus caldoxylosilyticus T20. Appl. Environ. Microbiol. 68: 2081-2084 [Abstract] [Full Text]  
• McGrath, J. W., Quinn, J. P. (2000). Intracellular Accumulation of Polyphosphate by the Yeast Candida humicola G-1 in Response to Acid pH. Appl. Environ. Microbiol. 66: 4068-4073 [Abstract] [Full Text]  
• McGrath, J. W., Hammerschmidt, F., Quinn, J. P. (1998). Biodegradation of Phosphonomycin by Rhizobium huakuii PMY1. Appl. Environ. Microbiol. 64: 356-358 [Abstract] [Full Text]