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EXPERIMENTAL SALMONELLOSIS III.

New Toxic Fraction (L) Obtained from Salmonella enteritidis and Its Immunological Properties

Department of Microbiology, School of Medicine, Gunma University, Maebashi, Japan

ABSTRACT

KAWAKAMI, MASAYA (Gunma University, Maebashi, Japan), NOBUTAKA OSAWA, AND SUSUMU MITSUHASHI. Experimental salmonellosis. III. New toxic fraction (L) obtained from Salmonella enteritidis and its immunological properties. J. Bacteriol. 86:872–879. 1963.—A method is described for the purification of the heat-labile toxins of a fully virulent strain, 116-54, of Salmonella enteritidis by ion-exchange chromatography. One component of the heat-labile toxin (L) was homogeneous, as evidenced by the results of the ultracentrifugal analysis and agar gel diffusion test. The mouse LD₅₀ was 1.3 µg, and chemical studies indicated that this toxin was a simple protein in nature. It was also evidenced by chemical and immunological tests that this toxin differs from the O antigen (lipopolysaccharide-protein complex).