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J Bacteriol. 1963 November; 86(5): 1037-1040
Copyright © 1963, The Williams & Wilkins Company. All Rights Reserved.

PREPARATION OF A COPPER-DEFICIENT MEDIUM FOR YEAST GROWTH

Department of Medicine, University of Utah College of Medicine, Salt Lake City, Utah

ABSTRACT

GIORGIO, ANTHONY J. (University of Utah, Salt Lake City), G. E. CARTWRIGHT, AND M. M. WINTROBE. Preparation of a copper-deficient medium for yeast growth. J. Bacteriol. 86:1037–1040. 1963.—A simple, rapid, and effective means was described for removing trace amounts of copper without removal of other growth nutrients from synthetic media. The complete synthetic medium minus added copper was batch-extracted with zinc dibenzyldithiocarbamate in carbon tetrachloride. The concentration of copper in extracted media was 0.3 to 1.7 µg per liter of media as compared with 8 µg per liter before extraction. Yeast growth in extracted media to which copper was added was comparable with growth in unextracted media. Yeast growth in copper-deficient media was significantly impaired. The growth requirement of two different strains of Saccharomyces cerevisiae was of the order of 10 µg of copper per liter of medium.