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J Bacteriol. 1963 November; 86(5): 1079-1083
Copyright © 1963, The Williams & Wilkins Company. All Rights Reserved.

TEMPERATURE-SENSITIVE DEXTRANSUCRASE SYNTHESIS BY A LACTOBACILLUS

Division of Bacteriology, College of Agriculture, Cornell University, Ithaca, New York

ABSTRACT

DUNICAN, L. K. (Cornell University, Ithaca, New York), AND H. W. SEELEY, JR. Temperature-sensitive dextransucrase synthesis by a lactobacillus. J. Bacteriol. 86:1079–1083. 1963.—Dextran synthesis was found to be temperature-dependent in Lactobacillus strain RWM-13. Dextran was not formed above 37 C, although growth of cells occurred up to 42 C. Logarithmically growing cells transferred from 30 C to 40 C ceased producing dextran while growth decreased nominally. An examination of the extracts of cells broken by sonic treatment showed that as the temperature of growth was increased above 37 C the production of dextransucrase decreased. By use of an inhibitor of invertase, 10^–4M AgNO₃, it was shown that invertase replaced dextransucrase activity at temperatures above 37 C. In contrast to dextransucrase in Leuconostoc mesenteroides, the enzyme in Lactobacillus strain RWM-13 was constitutive and thus resembled that of Streptococcus bovis. Thermosensitivity of dextransucrase synthesis has not been observed in Leuconostoc or Streptococcus.

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