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J Bacteriol. 1963 November; 86(5): 930-936
Copyright © 1963, The Williams & Wilkins Company. All Rights Reserved.

OCCURRENCE OF NUCLEOTIDES IN CULTURE FLUIDS OF MICROORGANISMS

V. EXCRETION OF ADENOSINE CYCLIC 3',5'-PHOSPHATE BY BREVIBACTERIUM LIQUEFACIENS SP. N.

Shionogi Research Laboratory, Shionogi & Co., Ltd., Fukushima-ku, Osaka, Japan

ABSTRACT

OKABAYASHI, TADASHI (Shionogi & Co., Ltd., Fukushima-ku, Osaka, Japan), AKIHIRO YOSHIMOTO, AND MISAO IDE. Occurrence of nucleotides in culture fluids of microorganisms. V. Excretion of adenosine cyclic 3',5'-phosphate by Brevibacterium liquefaciens sp. n. J. Bacteriol. 86:930–936. 1963.—Brevibacterium liquefaciens sp. n., when grown in a medium containing amino acids as the nitrogen source, excreted a considerable amount of an adenine ribonucleotide, which had not previously been noticed. The nucleotide was identified as adenosine cyclic 3',5'-phosphate by analysis, ultraviolet-absorption spectra, infrared-absorption spectra, paper chromatography, paper electrophoresis, and by comparison of behavior in hydrolysis by HCl, NaOH, and Ba(OH)₂; also, behavior in digestion with a crude enzyme preparation of adenosine cyclic 3',5'-phosphate phosphodiesterase was compared with that of an authentic sample. Preliminary examination of culture conditions revealed that, at least superficially, the substitution of DL-alanine for Casamino Acids (as nitrogen source) is one of the causes of the excretion of adenosine cyclic 3',5'-phosphate.

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