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MEDIUM FOR DIFFERENTIATING THE GRAM-NEGATIVE, NONFERMENTING BACILLI OF MEDICAL INTEREST

Immunobiology Section, U.S. Air Force School of Aerospace Medicine, Brooks Air Force Base, Texas

ABSTRACT

SELLERS, WALTER (U.S. Air Force School of Aerospace Medicine, Brooks Air Force Base, Tex.). Medium for differentiating the gram-negative, nonfermenting bacilli of medical interest. J. Bacteriol. 87:46–48. 1964.—An agar-slant medium is described for differentiating gram-negative, nonfermenting bacilli of medical interest. Differences in the ability of bacilli to grow anaerobically in the presence of nitrate, to produce pH changes, to produce N₂ from nitrite and nitrate (singly and in combination), to fluoresce, and to oxidize a drop of glucose solution to acid in the presence of high peptone concentrations were used in the development of the medium. Organisms differentiated by the medium include Pseudomonas aeruginosa, Bacterium anitratum, Mima polymorpha, and Vibrio alcaligenes or Alcaligenes faecalis.