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J Bacteriol. 1964 February; 87(2): 432-437
Copyright © 1964 by the American Society for Microbiology. All Rights Reserved.

FORMATION OF "PROTOPLASTS" OF STAPHYLOCOCCUS AUREUS WITH A FUNGAL N-ACETYLHEXOSAMINIDASE¹

^a Biochemical Research Section, Lederle Laboratories Division, American Cyanamid Company, Pearl River, New York

ABSTRACT

HASH, JOHN H. (Lederle Laboratories, Pearl River, N.Y.), MARCIA WISHNICK, AND PHILIP A. MILLER. Formation of "protoplasts" of Staphylococcus aureus with a fungal N-acetylhexosaminidase. J. Bacteriol. 87:432–437. 1964.—"Protoplasts" of Staphylococcus aureus were obtained when cells were treated with a fungal N-acetyl-hexosaminidase in hypertonic sucrose solution. The "protoplasts" were sensitive to osmotic shock, and were free of amino sugars and rigid cell walls. They were unable to regenerate cell walls and form colonies. They may be true protoplasts. Osmotic barriers in the "protoplasts" were preserved, and the "protoplasts" incorporated C¹⁴-L-alanine at about 60% of the rate of intact cells.

¹ A preliminary report on these data was presented at the 46th annual meeting of the Federation of American Societies for Experimental Biology, Atlantic City, N.J., April 1962.