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J Bacteriol. 1965 January; 89(1): 175-184
Copyright © 1965 American Society for Microbiology. All Rights Reserved.

Induction and Properties of a Temperate Bacteriophage from Bacillus stearothermophilus

N. E. Welker1 and L. Leon Campbell

a Department of Microbiology, University of Illinois, Urbana, Illinois

ABSTRACT

WELKER, N. E. (University of Illinois, Urbana), AND L. LEON CAMPBELL. Induction and properties of a temperate bacteriophage from Bacillus stearothermophilus. J. Bacteriol. 89:175–184. 1965.—Bacillus stearothermophilus 1503-4R growing at 55 C was induced to lyse either when 0.05 µg/ml of mitomycin C was added or when it was exposed to ultraviolet light for 30 sec. Lysis of the induced cultures occurred 45 to 60 min after induction. Phage were assayed on B. stearothermophilus 4S giving turbid plaques 0.05 to 0.3 cm in diameter. Noninduced cultures of 1503-4R spontaneously produced one phage per 2.8 x 106 bacterial cells. The optimal temperature for phage production and assay was found to be 55 C. B. stearothermophilus 1503-4R was immune to the isolated temperate phage (TP-1) and to a clear-plaque mutant phage (TP-1C), even when tested at phage mutliplicities of 100. TP-1 and TP-1C phage were identical morphologically having a head 65 mµ in diameter and a tail 240 mµ long and 12 mµ wide. TP-1C phage deoxyribonucleic acid (DNA) had an S20,w value of 24.1 and a calculated molecular weight of 1.21 x 107. DNA base compositions of TP-1 and TP-1C phage were identical (42% guanine + cytosine), but differed significantly from those of the lysogenic or indicator strains of B. stearothermophilus (50% guanine + cytosine). No unusual bases were detected in either the bacterial or phage DNA.

FOOTNOTES

1 Present address: Department of Biological Sciences, Northwestern University, Evanston, Ill.

J Bacteriol. 1965 January; 89(1): 175-184
Copyright © 1965 American Society for Microbiology. All Rights Reserved.





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