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J Bacteriol. 1965 January; 89(1): 193-197
Copyright © 1965 American Society for Microbiology. All Rights Reserved.

Experimental Salmonellosis IV. Lipid Content of Toxin L Obtained from Salmonella enteritidis

Department of Microbiology, School of Medicine, Gunma University, Maebashi, Japan

ABSTRACT

KAWAKAMI, MASAYA (Gunma University, Maebashi, Japan), AND SUSUMU MITSUHASHI. Experimental salmonellosis. IV. Lipid content of toxin L obtained from Salmonella enteritidis. J. Bacteriol. 89:193–197. 1965.—An improved method is described for the chromatographic purification of toxin L from virulent Salmonella enteritidis. Toxin L is heat-labile, and its toxicity is reduced by trypsin and by phospholipase A treatment. Chemical studies of chromatographically and ultracentrifugally homogeneous toxin preparations show that toxin L is a phosphorus-containing protein. The infrared-absorption spectrum of a petroleum ether extract of toxin L hydrolysate indicates that this material possesses a lipid component.