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J Bacteriol. 1965 January; 89(1): 240-244
Copyright © 1965 American Society for Microbiology. All Rights Reserved.

Electron Microscopic Observations on Actinophages for Streptomyces venezuelae

Department of Microbiology, University of Minnesota, Minneapolis, Minnesota

ABSTRACT

PAINTER, B. G. (University of Minnesota, Minneapolis), AND S. G. BRADLEY. Electron microscopic observations on actinophages for Streptomyces venezuelae. J. Bacteriol. 89:240–244. 1965.—Actinophages MSP2 and MSP8 were treated with various chemical agents to determine the phages' substructure. Normal MSP2 and MSP8, shadowed with uranium, or negatively stained with uranyl acetate or phosphotungstic acid, were tadpole-shaped. The average overall length of MSP2 was 245 mµ, and of MSP8, 206 mµ. The head width of both phages was approximately 58 mµ; the tail of MSP2 was 161 mµ long, and of MSP8, 137 mµ. Treatment of MSP2 and MSP8 with 7.5 M ammonium hydroxide at 56 C or 8 M urea at room temperature released some of the head contents, and revealed substructures in both the heads and the tails. The tails of phages treated with 7.5 M mercaptoethanol were destroyed.