This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Curtiss, R. Search for Related Content PubMed PubMed Citation Articles by Curtiss, R., III

 Previous Article  |  Next Article 

J Bacteriol. 1965 January; 89(1): 28-40
Copyright © 1965 American Society for Microbiology. All Rights Reserved.

Chromosomal Aberrations Associated with Mutations to Bacteriophage Resistance in Escherichia coli

^a Department of Microbiology, University of Chicago, Chicago, Illinois, and Biology Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee

ABSTRACT

CURTISS, ROY, III (University of Chicago, Chicago, Ill., and Oak Ridge National Laboratory, Oak Ridge, Tenn.). Chromosomal aberrations associated with mutations to bacteriophage resistance in Escherichia coli. J. Bacteriol. 89:28–40. 1965.—Ten types of mutants of Escherichia coli K-12 resistant to bacteriophage T₃ have been isolated, and several of these types have been studied genetically. Many of the /3,4,7, /3,4,7,, and /3,4,7,,pro^–_1,2 mutants were unstable, changing to complete sensitivity to T₄. The results with strains having /3,4,7,,pro^–_1,2 mutations were compatible with the hypothesis that this mutation caused a single break in the circular chromosome which prevented the normal association in the inheritance of the outside markers leu⁺ and lac⁺. When sensitivity to T₄ was regained, association in the inheritance of outside markers was restored, and the resulting /3,7,,pro^–_1,2 mutation behaved genetically as a deletion. The /3,7,,pro^–_1,2 and /3,4,7,,pro^–_1,2 mutations caused positive interference, inhibition of genetic recombination in regions adjacent to them, and the formation of unstable partial diploid recombinants. One group of /3,4,7, mutations did not occur in the leu to try region of the bacterial genome. Other /3,4,7, mutations in F^– bacteria prevented the joint inheritance of the outside markers lac⁺ and gal⁺, presumably by breakage of the circular chromosome. Hfr and F⁺ strains with /3,4,7, mutations at this locus were unable to conjugate; therefore, a complete genetic analysis of the effects of this /3,4,7, mutation could not be done.

¹ Present address: Biology Division, Oak Ridge National Laboratory, Oak Ridge, Tenn.

This article has been cited by other articles:

• (2008). Regulated programmed lysis of recombinant Salmonella in host tissues to release protective antigens and confer biological containment. Proc. Natl. Acad. Sci. USA 105: 9361-9366  
• Xin, W., Wanda, S.-Y., Li, Y., Wang, S., Mo, H., Curtiss, R. III (2008). Analysis of Type II Secretion of Recombinant Pneumococcal PspA and PspC in a Salmonella enterica Serovar Typhimurium Vaccine with Regulated Delayed Antigen Synthesis. Infect. Immun. 76: 3241-3254 [Abstract] [Full Text]  
• Bollen, W. S., Gunn, B. M., Mo, H., Lay, M. K., Curtiss, R. III (2008). Presence of Wild-Type and Attenuated Salmonella enterica Strains in Brain Tissues following Inoculation of Mice by Different Routes. Infect. Immun. 76: 3268-3272 [Abstract] [Full Text]  
• Lu, J., Sanchez, S., Hofacre, C., Maurer, J. J., Harmon, B. G., Lee, M. D. (2003). Evaluation of Broiler Litter with Reference to the Microbial Composition as Assessed by Using 16S rRNA and Functional Gene Markers. Appl. Environ. Microbiol. 69: 901-908 [Abstract] [Full Text]  
• Cote, C. K., Cvitkovitch, D., Bleiweis, A. S., Honeyman, A. L. (2000). A novel {beta}-glucoside-specific PTS locus from Streptococcus mutans that is not inhibited by glucose. Microbiology 146: 1555-1563 [Abstract] [Full Text]  
• Maturin, L Sr, Curtiss, R 3rd (1977). Degradation of DNA by nucleases in intestinal tract of rats. Science 196: 216-218 [Abstract]