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J Bacteriol. 1965 February; 89(2): 398-402
Copyright © 1965 American Society for Microbiology. All Rights Reserved.

Studies of Streptococcal Cell Walls VII. Carbohydrate Composition of Group B Cell Walls¹

^a Department of Biochemistry, Presbyterian-St. Luke's Hospital, and University of Illinois College of Medicine, Chicago, Illinois

ABSTRACT

WITTNER, MASAKO K. (Presbyterian-St. Luke's Hospital, Chicago, Ill.), AND JAMES A. HAYASHI. Studies of streptococcal cell walls. VII. Carbohydrate composition of group B cell walls. J. Bacteriol. 89:398–402. 1965.—Group B streptococcal cell walls contain 63% protein, 10% rhamnose, 18% hexose (mainly galactose, but also some glucose), 7% hexosamine (mainly glucosamine, but also galactosamine), and 3% muramic acid. The group and type antigens were extracted from isolated cell walls by acid treatment and enzymatic hydrolysis, and fractionated either with ethanol or on a diethylaminoethyl-cellulose column. Serological and chemical analyses of the fractions obtained in the two fractionation methods show that the group antigen is a rhamnose-rich polysaccharide and that the type antigen is rich in galactose and contains hexosamines.

¹ Taken in part from a thesis submitted in partial fulfillment of the requirements for the M.S. degree in the Graduate College, University of Illinois College of Medicine.