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J Bacteriol. 1965 March; 89(3): 574-582
Copyright © 1965 American Society for Microbiology. All Rights Reserved.

Effect of Enzymes on the Interaction of Enteroviruses with Living HeLa Cells¹

^a Langbord Virus Laboratory, Department of Microbiology, Hahnemann Medical College, Philadelphia, Pennsylvania

ABSTRACT

ZAJAC, IHOR (Hahnemann Medical College, Philadelphia, Pa.), AND RICHARD L. CROWELL. Effect of enzymes on the interaction of enteroviruses with living HeLa cells. J. Bacteriol. 89:574–582. 1965.—Eight crude enzyme preparations and two crystalline enzymes were tested for ability to inactivate coxsackie group B and poliovirus receptors on living HeLa cells. Receptor-destroying enzyme, erepsin, lysozyme, collagenase, proteinase, and cobra venom did not alter attachment of coxsackie B3 or poliovirus T1 to cells, whereas elastase prevented attachment of both viruses tested. Treatment of live cells with pancreatin or chymotrypsin rendered cells unable to attach group B coxsackie viruses, whereas cells treated with trypsin failed to attach poliovirus T1. In addition, chymotrypsin was found to release coxsackie B3 and poliovirus T1 from cell surfaces, whereas trypsin was unable to dissociate virus-cell union. These results indicate that cellular receptors for polioviruses differ from those for group B coxsackie-viruses. The finding that 1% solutions of enzymes will inactivate differentially the enteroviral receptors of HeLa cells, without altering cell viability, provides a useful approach for study of enterovirus receptors of live host cells.

² Research Career Development Award Grantee of the National Institute of Allergy and Infectious Diseases, U.S. Public Health Service.

¹ Presented in part as a preliminary report before the American Association of Immunologists, Chicago, 14 April 1964. The material in this paper represents in part the thesis submitted by I. Zajac in partial fulfillment of the requirements for the Ph.D. degree.

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