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J Bacteriol. 1965 March; 89(3): 626-629
Copyright © 1965 American Society for Microbiology. All Rights Reserved.

Isolation of Clostridium tetani from Soil

Department of Bacteriology, School of Medicine, Kanazawa University, Kanazawa, Japan

ABSTRACT

SANADA, ICHIRO (Kanazawa University, Kanazawa, Japan), AND SHOKI NISHIDA. Isolation of Clostridium tetani from soil. J. Bacteriol. 89:626–629. 1965.—The higher the temperatures applied to soil specimens, the weaker the toxigenicity of Clostridium tetani strains isolated from them. The glucose- and maltose-fermenting ability of these isolates was inversely proportional to their toxigenicity. The biological properties of atoxic strains were indistinguishable from those of C. tetanomorphum. Since a considerable number of toxic strains fermented glucose and maltose, these criteria are of doubtful value for differentiating C. tetani from C. tetanomorphum.